Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79392
Bai CB, et al., Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development. 2002 Oct;129(20):4753-61
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2676439
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Antibody:
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Anti-Nkx2.2
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2B:
embryonic day 10.5
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129 * Black Swiss * Swiss Webster
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Mutant Allele(s):
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Gli1tm2Alj/Gli1tm2Alj
Gli2tm1Alj/Gli2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1tm1Mps
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
6B
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Authors state ventral spinal cord markers expand dorsally compared with wild-type embryos at E10.5.
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Specimen
6F:
embryonic day 10.5
(more )
Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
6F
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Expression in V3 interneurons adjacent to floor plate cells.
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Specimen
6J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
Ptch1tm1Mps/Ptch1tm1Mps
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Trace |
Regionally restricted |
6J
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Expression is greatly reduced; the number of V3 interneurons is greatly reduced; only a small number of V3 interneurons remained specifically in the ventral midline.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Trace |
Regionally restricted |
7B
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Expression is greatly reduced in the ventral midline.
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Analysis Tools
