Probe:	Jun probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: posterior pro-rhombomere	Present		1A a

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		1A b
TS13: head mesenchyme	Present		1A b
TS13: heart	Present		1A b
TS13: hindgut diverticulum	Present		1A b
TS13: posterior pro-rhombomere	Present		1A b
TS13: neural tube	Present	Regionally restricted	1A b	Expression is in the caudal dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	1A c	Expression is posterior.
TS14: foregut	Present		1A c
TS14: head mesenchyme	Present		1A c
TS14: heart	Present		1A c
TS14: hindgut diverticulum	Present		1A c
TS14: trunk somite	Present		1A c
TS14: neural tube	Present	Regionally restricted	1A c	Expression is in the caudal dorsal neural tube.
TS14: blood vessel	Present	Regionally restricted	1A c	Expression is in intersomitic blood vessels.

Structure	Level	Pattern	Image	Note
TS12: posterior pro-rhombomere neural fold	Present	Regionally restricted	1A a'	Expression is restricted to the middle of the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS13: septum transversum	Present		1A b'

Structure	Level	Pattern	Image	Note
TS14: trunk sclerotome	Present		1A c'

Structure	Level	Pattern	Image	Note
TS11: future spinal cord neural plate	Absent		1B d

Structure	Level	Pattern	Image	Note
TS12: posterior pro-rhombomere	Present	Regionally restricted	1B e	Expression is in a narrow stripe of cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 5	Present		1B f
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	1B f	Expression is in prospective rhombomere 6.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	1B g	Expression extends into prospective prorhombomere 6.
TS13: rhombomere 5	Present		1B g

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	1B h	Expression extends into the region of prospective prorhombomere 6.
TS13: rhombomere 5	Present		1B h

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	2A , 2B	Expression extends into prospective rhombomere 6. Expression in this mutant is the same as in wild-type embryos.
TS13: rhombomere 5	Present	Regionally restricted	2A , 2B	Expression extends into prospective rhombomere 6. Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Absent		2D , 2C
TS13: rhombomere 5	Absent		2D , 2C

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	2E , 2F	Expression in this mutant is the same as in wild-type embryos.
TS13: rhombomere 5	Present	Regionally restricted	2E , 2F	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	2H , 2G	Expression in this mutant is the same as in wild-type embryos.
TS13: rhombomere 5	Present	Regionally restricted	2H , 2G	Expression in this mutant is the same as in wild-type embryos.