Probe:	Shh cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	It is possible that another Shh probe (MGI:1929246) was used in this assay.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3G	Expression was detected in 2 ventrolateral strips and absent from the midline.
TS15: future hindbrain	Present	Regionally restricted	3G	Expression was detected in the floor plate.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3I	Expression was detected in the ventral midline at the rostral limit of the forebrain.
TS15: branchial arch endoderm	Present	Regionally restricted	3I	Expression was detected in the pharyngeal endoderm.
TS15: future hindbrain	Present	Regionally restricted	3I	Expression was detected in the floor plate.
TS15: notochord	Present		3I

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	3K	Expression was restricted ventrally.
TS15: notochord	Present		3K

Structure	Level	Pattern	Image	Note
TS16: neural tube floor plate	Present		4B
TS16: notochord	Present		4B
TS16: forelimb bud mesenchyme	Present	Regionally restricted	4C , 4B	Expression was detected posteriorly.
TS16: future brain	Present	Regionally restricted	4B	Expression was detected in the floorplate.

Structure	Level	Pattern	Image	Note
TS19: gut	Present		4F
TS19: neural tube floor plate	Present		4F
TS19: notochord	Present		4F
TS19: footplate	Present	Regionally restricted	4F	Expression was detected distally and posteriorly.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Strong	Regionally restricted		Expression was posterior and distal.
TS17: hindlimb bud mesenchyme	Strong	Regionally restricted		Expression was posterior and distal.

Structure	Level	Pattern	Image	Note
TS20: limb	Absent