Probe:	Shh cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	It is possible that another Shh probe (MGI:1929246) was used in this assay.

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS11: notochordal process	Present	Regionally restricted	2C	Expression was detected in the midline mesoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	2E	Expression was detected in the node.
TS12: notochord	Present	Regionally restricted	2E	Expression was detected in the head process underlying the presumptive brain.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	2F , 2G	Expression was detected in the node.
TS12: notochord	Present	Regionally restricted	2G , 2F	Expression was detected in the head process underlying the presumptive brain.

Structure	Level	Pattern	Image	Note
TS12: notochord	Present		2J , 2I
TS12: primitive streak	Present	Regionally restricted	2I , 2J	Expression was detected in the node.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	2N , 2M	Expression was present in the ventral midline.
TS13: notochord	Present		2M , 2N
TS13: gut endoderm	Present	Regionally restricted	2N , 2M	Expression was detected in the caudal endoderm in 2 ventrolateral strips of cells.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	2O	Expression was ventrally restricted.
TS13: neural tube floor plate	Present		2O
TS13: notochord	Present		2O
TS13: future hindbrain	Present	Regionally restricted	2O	Expression was present in the ventral midline, the presumptive floor plate.
TS13: future midbrain	Present	Regionally restricted	2O	Expression was detected ventrolaterally.
TS13: gut endoderm	Present	Regionally restricted	2O	Expression was present in the caudal endoderm.

Structure	Level	Pattern	Image	Note
TS14: hindgut diverticulum	Present	Regionally restricted	2Q	Expression was detected ventrally.
TS14: future hindbrain	Strong	Regionally restricted	2Q	Expression was detected in the floor plate.
TS14: neural tube floor plate	Strong		2Q
TS14: future diencephalon	Strong	Regionally restricted	2Q	Expression was detected ventrally.
TS14: future midbrain	Strong	Regionally restricted	2Q	Expression was detected ventrally.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	2S	Expression was detected ventrally.
TS15: diencephalon	Present	Regionally restricted	2S	Expression was detected in the rostral half of the diencephalon in ventrolateral strips but was absent from the floor plate. The strips merged in the rostral-most diencephalon to the ventral floor plate.
TS15: future midbrain floor plate	Present		2S
TS15: neural tube floor plate	Present		2S
TS15: notochord	Present		2S
TS15: future hindbrain	Present	Regionally restricted	2S	Expression was detected in the floor plate.
TS15: branchial arch endoderm	Present	Regionally restricted	2S	Expression was detected in the pharyngeal endoderm.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted		Expression was detected in the notochord.
TS21: medulla oblongata floor plate	Present
TS21: metencephalon floor plate	Present
TS21: midbrain floor plate	Present
TS21: spinal cord floor plate	Present

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	3A	Expression was detected at the midline.
TS12: notochord	Present		3A

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present	Regionally restricted	3C	Expression was detected in endoderm immediately beneath the forebrain.
TS12: notochord	Present		3C

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present		3E
TS13: notochord	Present		3E
TS13: hindgut diverticulum endoderm	Present	Regionally restricted	3E	Expression was detected in the caudal endoderm.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	4A	Expression was detected in a small group of posterior cells.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Strong	Regionally restricted	4D	Expression was detected in a strip of distal mesenchymal cells 6 cells wide that associated with the overlying ectoderm.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Strong	Regionally restricted	4E	Expression was detected in a strip of distal mesenchymal cells 6 cells wide that associated with the overlying ectoderm.