Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78220
Zhang Z, et al., Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis. Development. 2002 Sep;129(17):4135-46
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2383974
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2J:
embryonic day 12.5
(more  )
Specimen
2J:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf epithelium |
Present |
Regionally restricted |
2J
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Expression in epithelium of anterior palatal shelves, most prominent in medial edge epithelium.
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Specimen
2K:
embryonic day 13.5
(more )
Specimen
2K:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf epithelium |
Present |
Regionally restricted |
2K
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Expression in medial edge epithelium of anterior palatal shelves.
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Specimen
2L:
embryonic day 13.5
(more )
Specimen
2L:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf epithelium |
Present |
Regionally restricted |
2L
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Authors describe expression in oral side epithelium, where rugae would form; there is no expression in medial edge epithelium of the posterior palatal shelf.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae * CD-1
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf epithelium |
Present |
Regionally restricted |
3D
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Authors report expression is unaltered in mutant medial edge epithelium.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S4/SvJae * CD-1
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools