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Gene Expression Data
Assay Details
Assay
Reference: J:76131 Holley SJ, et al., Complementary Expression of IGF-II and IGFBP-5 during Anterior Pituitary Development. Dev Biol. 2002 Apr 15;244(2):319-28
Assay type: RNA in situ
MGI Accession ID: MGI:2179264
Gene symbol: Igf2
Gene name: insulin-like growth factor 2
Probe: Igf2 cDNA9
Probe preparation: Antisense RNA
Assay notes: Probes were labeled with 32P or 35S.
Results
Specimen E10.5: embryonic day 10.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS17: Rathke's pouch Strong Regionally restricted 5_IGF-II e10.5 Expression in the pouch epithelium and bordering ectoderm.
TS17: oral epithelium Strong Regionally restricted 5_IGF-II e10.5 Expression in the pouch epithelium and bordering ectoderm.
Specimen E11.5: embryonic day 11.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS19: Rathke's pouch Strong Regionally restricted 5_IGF-II e11.5 Expression in the dorsal region pouch epithelium.
Specimen E12.5: embryonic day 12.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS20: remnant of Rathke's pouch Present Regionally restricted 5_IGF-II e12.5 Expression in a more dorsal region and around the pouch itself.
Specimen E14.5: embryonic day 14.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS22: adenohypophysis Weak 6_IGF-II e14.5
TS22: pituitary gland Strong Regionally restricted 6_IGF-II e14.5 Expression in the surrounding tissues.
Specimen E18.5: embryonic day 18.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS26: adenohypophysis Absent 6_IGF-II e18.5
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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