Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73775
Byrd N, et al., Hedgehog is required for murine yolk sac angiogenesis. Development. 2002 Jan;129(2):361-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2177410
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Antibody:
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Anti-smooth muscle alpha-Actin (clone 1A4)
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Assay notes:
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The Ihh mutation was either generated in CJ7 or R1 ES cells and these were injected into C57BL/6 mice. Chimeric males were mated to C57BL/6, 129/Sv, or Swiss Webster females. Heterozygotes were maintained on a non-specified background.
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Results
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Specimen
1E:
embryonic day 10.5; Ptch1tm1Mps/Ptch1 +
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: extraembryonic component |
Present |
Regionally restricted |
1E
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Expression in the outermost layer surrounding blood vessels as well as the mesothelial cells between vessels of the yolk sac.
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Specimen
6B, +/-:
embryonic day 11.5; Ihhtm1Amc/Ihh +
(more )
Specimen
6B, +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihh+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B, -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown, +/-:
embryonic day 9.5; Ihhtm1Amc/Ihh +
(more )
Specimen
Not shown, +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac |
Weak |
Regionally restricted |
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There is faint staining around the blood vessels in the yolk sac.
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Specimen
Not shown, -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac |
Weak |
Regionally restricted |
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There is faint staining around the blood vessels in the yolk sac.
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Specimen
Not shown, +/-:
embryonic day 10.5; Ihhtm1Amc/Ihh +
(more )
Specimen
Not shown, +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihh+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: yolk sac |
Present |
Regionally restricted |
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There is staining around the blood vessels in the yolk sac.
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Specimen
Not shown, -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: yolk sac |
Weak |
Regionally restricted |
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There is staining around the blood vessels in the yolk sac; there is less seen in the homozygotes compared to the heterozygotes.
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Analysis Tools