Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72380
Makino S, et al., A Spontaneous Mouse Mutation, mesenchymal dysplasia (mes), Is Caused by a Deletion of the Most C-Terminal Cytoplasmic Domain of patched (ptc). Dev Biol. 2001 Nov 1;239(1):95-106
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2153856
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixation for the whole mounted embryos in this assay was in 3.5% paraformaldehyde.
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Results
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Specimen
3D:
embryonic day 12.0; Ptch1tm1Mps/Ptch1 +
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
3D
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Expression is in the posterior mesenchyme. Expression in this mutant is the same as in wild-type embryos.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/Fe * C57BL/6 * CBA/J
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Mutant Allele(s):
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Ptch1mes/Ptch1tm1Mps
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
3E
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There is ectopic expression at the anterior margin ot the limb bud. This expression is very weak in some cases. There is also normal expression in the posterior.
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Specimen
5E:
embryonic day 11.5; Ptch1tm1Mps/Ptch1 +
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/Fe * C57BL/6 * CBA/J
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Mutant Allele(s):
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Ptch1mes/Ptch1tm1Mps
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
