Antibody:	anti-Foxd3 polyclonal
Detection system:	Secondary antibody
Assay notes:	Species-specific secondary antibodies were conjugated to Cy3, Cy2 or FITC.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted		Authors report expression at both cervical and trunk levels in a domain that spans the dorsal midline where neural crest progenitors are located.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Regionally restricted	1A	At thoracic levels, expression in dorsal midline cells; expression in premigratory neural crest cells.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	1B , 1C	At cervical levels, expression in dorsal midline cells; expression in presumptive premigratory neural crest cells and in neural crest cells emigrating from the dorsal neural tube.
TS15: neural crest	Present	Regionally restricted	1B , 1C	At cervical levels, expression in dorsal midline cells; expression in presumptive premigratory neural crest cells and in neural crest cells emigrating from the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	1D	Majority of expressing neural crest cells located at the periphery of ganglia.
TS17: dorsal root ganglion	Weak	Regionally restricted	1D	Low levels of expression in a subset of cells in the dorsal root ganglia; not expressed in postmitotic sensory neurons.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	1E	Majority of expressing cells located at the periphery of ganglia.
TS17: sympathetic nervous system	Absent		1E	Not expressed in postmitotic sympathetic neurons of spinal ganglia.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present	Regionally restricted	1F	Expression in cells encompassing and within the dorsal root ganglion.
TS17: migrating neural crest	Present	Regionally restricted	1F	Expression in cells encompassing and within the dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	1G	Expression maintained in Schwann cell precursors that envelope axons of the peripheral nerves.

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	1H	Expression in melanocyte precursors migrating beneath the ectoderm.

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	1I	Expression in neural crest-derived cells in both dorsolateral (melanocyte precursors migrating beneath the ectoderm) and ventral (cells enveloping the dorsal root ganglia) migration pathways.

Structure	Level	Pattern	Image	Note
TS22: trunk mesenchyme	Present	Regionally restricted		Expression in cells surrounding the peripheral ganglia and axon tracts.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present		2C	Expression in neural crest cells generated along the length of the neural tube from midbrain/hindbrain border to the tail.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present		2D	Expression in neural crest cells generated along the length of the neural tube from midbrain/hindbrain border to the tail.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		2G	Expression in hindbrain-derived neural crest cells at levels that contribute to cranial ganglia V, VII and IX.

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Absent		2H	There is no expression in trunk neural crest cells in caudal regions where dorsal root ganglia and sympathetic ganglia are missing, although there is expression more anteriorly.

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2I	At anterior cervical neural tube level, expression in migrating neural crest cells.
TS16: peripheral nervous system	Present	Regionally restricted	2I	Expression in postmitotic ventral interneurons.

Structure	Level	Pattern	Image	Note
TS16: peripheral nervous system	Present	Regionally restricted	2J	Expression in postmitotic ventral interneurons.