Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72300
Dottori M, et al., The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development. 2001 Nov;128(21):4127-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2152661
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Antibody:
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rat anti-Pax3
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Detection system:
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Secondary antibody
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Assay notes:
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Species-specific secondary antibodies were conjugated to Cy3, Cy2 or FITC.
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Note: Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural crest |
Present |
Regionally restricted |
1A
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At thoracic levels, expression in dorsal midline and premigratory neural crest cells.
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| TS15: migrating neural crest |
Present |
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1A
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At thoracic levels, expression in migrating neural crest cells.
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Specimen
1B,1C:
embryonic day 9.5
(more )
Note: Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
Specimen
1B,1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural crest |
Present |
Regionally restricted |
1B
,
1C
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At cervical levels, expression in dorsal midline and premigratory neural crest cells.
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| TS15: migrating neural crest |
Present |
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1B
,
1C
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At cervical levels, expression in migrating neural crest cells.
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Specimen
1I:
embryonic day 12.5
(more )
Note: Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax3; green - Foxd3 (assay MGI:2152658).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: migrating neural crest |
Present |
Regionally restricted |
1I
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Expression in cells enveloping the dorsal root ganglia; also in neural crest cells migrating along a dorsolateral pathway beneath the skin.
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Analysis Tools