Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64588
Toresson H, et al., Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for pax6 and gsh2. Development. 2000 Oct;127(20):4361-71
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2150341
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Probe:
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neurogenin2 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2E:
embryonic day 11.5
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
2E
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Expressed in cortical progenitors.
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Specimen
2K:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2K
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Ectopic expression in lateral ganglionic eminence (LGE) progenitors.
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Authors report alterations in expression in the LGE.
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Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
5G
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Significant downregulation throughout the cortex; only in a small number of LGE progenitors; no expression in the lateral-most edge of the LGE and ventrolateral cortex.
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Specimen
6E:
embryonic day 14.0
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6J:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
6J
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Only in a small number of LGE progenitors; no expression in the dorsolateral cortical ventricular zone (VZ) where GSH2 is ectopic.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Absent |
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No longer ectopically expressed in LGE progenitors.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Absent |
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No expression in dorsolateral cortex.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
7D
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Downregulated in cortex; only in a small number of LGE progenitors; similar to that in Sey homozygous mutants.
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Analysis Tools