Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62985
Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149750
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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clone 39.4D5
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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This antibody recognizes Isl2 weakly and the authors use this antibody so they can differentiate between medial and lateral motor columns.
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Results
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Specimen
4A:
embryonic day 14.5
(more  )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4A:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4A
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
4B:
embryonic day 14.5
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4B:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4B
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
4C:
embryonic day 14.5
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4C
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
4D:
embryonic day 14.5
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4D
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
4E:
embryonic day 14.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4E:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Gelatin
|
|
Note:
|
Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4E
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns is slightly reduced.
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Specimen
4F:
embryonic day 14.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4F:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
|
|
Note:
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Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4F
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns is slightly reduced.
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Specimen
4G:
embryonic day 14.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4G:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
|
Note:
|
Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4G
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns is slightly reduced.
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Specimen
4H:
embryonic day 14.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
4H:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
|
Note:
|
Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
4H
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns is slightly reduced.
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Specimen
8F:
embryonic day 14.5
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
8F:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
|
Note:
|
Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
8F
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
8G:
embryonic day 14.5
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
8G:
(close )
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Genetic Background:
|
either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
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Sex:
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Not Specified
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Type:
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section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
|
Note:
|
Specimens were embedded in albumin/gelatin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
8G
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Expression in medial (mLMC) but not lateral (lLMC) lateral motor columns.
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Specimen
not shown:
embryonic day 15.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
|
Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basal columns |
Present |
Regionally restricted |
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General reduction in the number of expressing neurons in segments L2 to L5.
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Specimen
not shown:
embryonic day 16.5; Epha4tm1Pch/ Epha4tm1Pch
(more )
Note: Specimens were embedded in albumin/gelatin.
Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Gelatin
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|
Note:
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Specimens were embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: basal columns |
Present |
Regionally restricted |
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General reduction in the number of expressing neurons in segments L2 to L5.
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