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Gene Expression Data
Assay Details
Assay
Reference: J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type: RNA in situ
MGI Accession ID: MGI:2148284
Gene symbol: En2
Gene name: engrailed 2
Probe: En2 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3I: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Graded 3I Gradient of expression over the caudal mesencephalon and rhombomere 1 (r1).
TS15: rhombomere 1 Present Graded 3I Gradient of expression over the caudal mesencephalon and rhombomere 1 (r1).
Specimen 3J: embryonic day 9.5; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 3J Expanded expression rostral to the isthmic constriction; gradient is less distinct.
TS15: future hindbrain Present 3J Enlarged regions of expression caudal to isthmic constriction and between posterior terminus of expression and otic vesicle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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