Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:36832
Stoykova A, et al., Forebrain patterning defects in Small eye mutant mice. Development. 1996 Nov;122(11):3453-65
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2148110
|
|
Gene symbol:
|
Otx2
|
|
Gene name:
|
orthodenticle homeobox 2
|
|
Probe:
|
Otx2 cDNA1
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
2C:
embryonic day 12.5
(more  )
Specimen
2C:
(close  )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
2C
|
|
| TS20: telencephalon |
Absent |
|
2C
|
No expression in wild-type cortex.
|
| TS20: thalamus |
Present |
Regionally restricted |
2C
|
Expression in ventral thalamus, including the dorsalmost region (Fig 2I). Specific labelling in eminential thalami (EMT) primordia and very rostrodorsal part of ventral thalamus.
|
Specimen
2F:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2I:
embryonic day 12.5
(more )
Specimen
2I:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
2I
|
|
| TS20: telencephalon |
Absent |
|
2I
|
No expression in wild-type cortex.
|
| TS20: thalamus |
Present |
Regionally restricted |
2I
|
Expression in ventral thalamus, including the dorsalmost region (Fig 2I). Specific labelling in eminential thalami (EMT) primordia and very rostrodorsal part of ventral thalamus.
|
Specimen
2L:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2O:
embryonic day 12.5
(more )
Specimen
2O:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
2O
|
|
| TS20: thalamus |
Present |
Regionally restricted |
2O
|
Expression in ventral thalamus, including the dorsalmost region (Fig 2I). Specific labelling in eminential thalami (EMT) primordia and very rostrodorsal part of ventral thalamus.
|
Specimen
2R:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2U:
embryonic day 12.5
(more )
Specimen
2U:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
2U
|
|
| TS20: thalamus |
Present |
Regionally restricted |
2U
|
Expression in ventral thalamus, including the dorsalmost region (Fig 2I). Specific labelling in eminential thalami (EMT) primordia and very rostrodorsal part of ventral thalamus.
|
Specimen
2X:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
Present |
Regionally restricted |
|
Abundant in lateral areas including reticular thalamic nucleus and ventral lateral geniculate body.
|
|
Analysis Tools