Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48569
Grove EA, et al., The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development. 1998 Jun;125(12):2315-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136883
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Gene symbol:
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Ttr
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Gene name:
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transthyretin
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Probe:
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rat transthyretin cDNA
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.
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Results
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Specimen
4B:
embryonic day 10.5
(more  )
Specimen
4B:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Absent |
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4B
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No differentiated choroid plexus epithelium can be detected by Ttr expression in the medial wall.
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Specimen
4E:
embryonic day 11.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
4E
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Expression marks differentiating telencephalic choroid plexus epithelium.
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Specimen
2A, 2B:
embryonic day 12.5
(more )
Specimen
2A, 2B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Absent |
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2C
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No expression in the cortical hem (at the boundary between cortex and choroid plexus epithelium) or in the junctional epithelium (between the choroid plexus epithelium and hem).
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| TS20: choroid plexus |
Strong |
Regionally restricted |
2C
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Strong expression in choroid plexus epithelium.
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Specimen
7A:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7F:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Absent |
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7F
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In non-exencephalic homozygous mutants, no expression at the base of the medial telencephalic wall.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Absent |
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Five non-exencephalic homozygous mutants did not show expression in the medial telencephalon.
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Analysis Tools