Hand1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:59338 Clouthier DE, et al., Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Dev Biol. 2000 Jan 1;217(1):10-24
Assay type:	RNA in situ
MGI Accession ID:	MGI:2135645
Gene symbol:	Hand1
Gene name:	heart and neural crest derivatives expressed 1

Probe:	eHand probe9
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3C +/+ ©

Specimen 3C +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen to be consistent with other annotations from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	3C +/+	Expression is in the distal 1/3.

3D +/+ ©

Specimen 3D +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen to be consistent with other annotations from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	3D +/+	Expression is in the distal 1/3.

3C -/- ©

Specimen 3C -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen to be consistent with other annotations from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Absent		3C -/-

3D -/- ©

Specimen 3D -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen to be consistent with other annotations from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Absent		3D -/-

3G +/+ ©

Specimen 3G +/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Moderate	Regionally restricted	3G +/+	Expression is in the distal 1/3.

3H +/+ ©

Specimen 3H +/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Moderate	Regionally restricted	3H +/+	Expression is in the distal 1/3.

3G -/- ©

Specimen 3G -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Trace	Regionally restricted	3G -/-	Expression is in the extreme distal edge.

3H -/- ©

Specimen 3H -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Trace	Regionally restricted	3H -/-	Expression is in the extreme distal edge.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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