Probe:	pHOX8
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe was shortened by limited alkaline hydrolysis to around 100 bases.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present		1B	Expression in the mesenchyme adjacent to, and anterior to, an epithelial thickening.
TS17: 1st branchial arch maxillary component mesenchyme	Present		1B	Expression in the mesenchyme adjacent to, and anterior to, an epithelial thickening.
TS17: 1st branchial arch mandibular component ectoderm	Weak	Regionally restricted	1B	Expression from the tip of the process into the anterior edge of the dental epithelial thickening.
TS17: 1st branchial arch maxillary component ectoderm	Weak	Regionally restricted	1B	Expression from the tip of the process into the anterior edge of the dental epithelial thickening.
TS17: 2nd branchial arch	Present		1B	Negligible expression in the anterior region of the second branchial arch.

Structure	Level	Pattern	Image	Note
TS20: maxillary process epithelium	Present	Regionally restricted	1D	Expression in the mesenchyme and buccal external enamel epithelium of the upper tooth and in the epithelial bud in the lower.
TS20: maxillary process mesenchyme	Present	Regionally restricted	1D	Expression in the mesenchyme and buccal external enamel epithelium of the upper tooth and in the epithelial bud in the lower.
TS20: mandibular process	Present	Regionally restricted	1D	Expression in the mesenchyme and buccal external enamel epithelium of the upper tooth and in the epithelial bud in the lower.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar enamel organ	Present		2C	Expression in the enamel knot and adjacent internal and external enamel epithelium.
TS22: enamel knot	Present		2C
TS22: inner enamel epithelium	Present		2C
TS22: outer enamel epithelium	Present		2C
TS22: lower jaw molar mesenchyme	Present		2C	Expression was highest in the mesenchyme condensed around or inside the enamel organ.
TS22: lower jaw molar dental papilla	Present		2C

Structure	Level	Pattern	Image	Note
TS24: lower jaw molar mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar mesenchyme	Present