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Gene Expression Data
Assay Details
Assay
Reference: J:2360 Moase CE, et al., N-CAM alterations in splotch neural tube defect mouse embryos. Development. 1991 Nov;113(3):1049-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1934932
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: anti-NCAM polyclonal
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Regionally restricted 1A Staining in the neuroepithelium of the closing neural tube in the posterior region.
TS14: trunk mesenchyme Present Regionally restricted 1A Staining in the apical area of the mesoderm.
TS14: notochord Weak 1A
TS14: gut Absent 1A
TS14: surface ectoderm Absent 1A

Specimen 1B: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Regionally restricted 1B In a region were the neural folds begin to fuse staining is most intense in the lateral portions of the neural tube.
TS14: trunk mesenchyme Present Regionally restricted 1B Less staining in the mesoderm surrounding the neural tube.

Specimen 1C: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Regionally restricted 1C Staining is most intense in the lateral portions of the neural tube in a region where the neural folds appose one another just prior to fusion.
TS14: trunk mesenchyme Present Regionally restricted 1C Staining is in the mesoderm surrounding the neural tube.

Specimen 1D: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: future spinal cord neural plate Present Regionally restricted 1D Staining in the open caudal region; even distribution throughout the neuroepithelial tissue.
TS14: neural tube Present Regionally restricted 1D Staining in the open caudal region; even distribution throughout the neuroepithelial tissue.

Specimen 2B: embryonic day 8.5; Pax3Sp/Pax3Sp (more )
Note: 9 somites.
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 2B Section through the hindbrain region showing the foregut. Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain region.
TS13: future prosencephalon Present Regionally restricted 2B Section through the hindbrain region showing the foregut. Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain region.

Specimen 2C: embryonic day 8.5; Pax3Sp/Pax3Sp (more )
Note: 9 somites.
Structure Level Pattern Image Note
TS13: neural tube Present 2C Open caudal region of the embryo. Staining in the neuroepithelium.

Specimen 2D: embryonic day 8.5 (more )
Note: 11 somites.
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 2D Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.
TS13: future prosencephalon Present Regionally restricted 2D Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.

Specimen 2E: embryonic day 8.5 (more )
Note: 11 somites.
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 2E Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.
TS13: future prosencephalon Present Regionally restricted 2E Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.

Specimen 2F: embryonic day 8.5 (more )
Note: 11 somites.
Structure Level Pattern Image Note
TS13: neural tube Present Regionally restricted 2F Open caudal region of the embryo. Expression in the lateral portions of the neural tube.

Specimen 3A: embryonic day 9.0; Pax3Sp/Pax3Sp (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Strong 3A Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Specimen 3B: embryonic day 9.0; Pax3Sp/Pax3Sp (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Strong 3B Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Specimen 3C: embryonic day 9.0; Pax3Sp/Pax3Sp (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Strong 3C Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Specimen not shown: embryonic day 9.5; Pax3Sp/Pax3Sp (more )
Note: 16 - 24 somites.
Structure Level Pattern Image Note
TS15: neural tube Strong Strong staining along the lateral portions of the neural tube.

Specimen 3D: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present 3D Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Specimen 3E: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present 3E Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Specimen 3F: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present 3F Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Specimen not shown: embryonic day 9.5 (more )
Note: 16 - 24 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Staining is reduced compared to Sp-/- embryos.

Specimen not shown: embryonic day 9.0; Pax3Sp/Pax3+ (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Staining is similar to wild-type but the heterozygotes showed more variability.

Specimen not shown: embryonic day 9.0; Pax3Sp-d/Pax3+ (more )
Note: 17-25 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Specimen not shown: embryonic day 9.0; Pax3Sp-d/Pax3+ (more )
Note: 17-25 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Specimen not shown: embryonic day 9.0; Pax3Sp-d/Pax3Sp-d (more )
Note: 17-25 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Specimen not shown: embryonic day 9.0; Pax3Sp-d/Pax3Sp-d (more )
Note: 17-25 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Less dramatic differences between mutant and non-mutant embryos in staining patterns.


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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory