Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:21617
Gunther T, et al., Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development. 1994 Nov;120(11):3119-30
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1933418
|
|
Gene symbol:
|
Shh
|
|
Gene name:
|
sonic hedgehog
|
|
Probe:
|
Shh cDNA3
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
6A:
embryonic day 12.5
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: notochord |
Present |
|
6A
|
Expression in the notochord and ventral spinal cord region (presumptive floor plate).
|
| TS20: spinal cord floor plate |
Present |
|
6A
|
Expression in the notochord and ventral spinal cord region (presumptive floor plate).
|
Specimen
6B:
embryonic day 12.5; Rab23opb/ Rab23opb
(more )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
6B:
(close )
|
Genetic Background:
|
involves: C57BL/6 * NMRI
|
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: notochord |
Present |
|
6B
|
Expression in the notochord and vetral spinal cord region (presumptive floor plate); the area of Shh-positive cells marking the floor plate was much larger than in wild-type.
|
| TS20: spinal cord floor plate |
Present |
|
6B
|
Expression in the notochord and vetral spinal cord region (presumptive floor plate); the area of Shh-positive cells marking the floor plate was much larger than in wild-type.
|
|
Analysis Tools
