Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57670
Mishina Y, et al., Multiple roles for activin-like kinase-2 signaling during mouse embryogenesis. Dev Biol. 1999 Sep 15;213(2):314-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1931754
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T cDNA9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3. A. Left WT:
embryonic day 8.0
(more  )
Specimen
3. A. Left WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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Specimen
3. A. Center -/- 1:
embryonic day 8.0; Acvr1tm1Bhr/ Acvr1tm1Bhr
(more )
Note: 4 of 14 homozygous mutant expressed Brachury.
Specimen
3. A. Center -/- 1:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Acvr1tm1Bhr/Acvr1tm1Bhr
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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Note:
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4 of 14 homozygous mutant expressed Brachury.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
Regionally restricted |
3A center
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Four of 14 abnormal embryos expressed Brachyury, but the expression domain was restricted to a small posterior region.
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Specimen
3. A. Right -/- 2:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Acvr1tm1Bhr/Acvr1tm1Bhr
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Absent |
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3A right
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Most of the abnormal embryos did not express Brachyury, as shown here.
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Specimen
E7.0 WT:
embryonic day 7.0
(more )
Specimen
E7.0 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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Specimen
E7.0 -/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Acvr1tm1Bhr/Acvr1tm1Bhr
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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Analysis Tools