Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65601
Garel S, et al., Control of the migratory pathway of facial branchiomotor neurones. Development. 2000 Dec;127(24):5297-307
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1929003
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Gene symbol:
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Phox2b
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Gene name:
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paired-like homeobox 2b
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Probe:
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Phox2b probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 11.25
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
2A
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Expression is in migrating fbm neurons.
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| TS18: rhombomere 5 |
Present |
Regionally restricted |
2A
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Expression is in migrating fbm neurons.
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| TS18: rhombomere 6 |
Present |
Regionally restricted |
2A
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Expression is in fbm neurons just entering rhombomere 6.
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Specimen
2B:
embryonic day 12.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
2B
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Expression is in facial bm neurons located in rhombomere 4,5 and 6.
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Specimen
3A:
embryonic day 12.5; Mafbkr/Mafb +
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafb+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3A
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Expression is in dorsal migrating facial bm neurons located in rhombomere 4,5 and 6.
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Specimen
3A':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3A'
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The expressing fbm neurons form abnormally thick streams during dorsal migration due to an abnormal rhombomere 6 in this mutant.
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Specimen
6D:
embryonic day 11.25
(more )
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
6D
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Expression is in migrating fbm neurons.
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| TS18: rhombomere 5 |
Present |
Regionally restricted |
6D
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Expression is in migrating fbm neurons.
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| TS18: rhombomere 6 |
Present |
Regionally restricted |
6D
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Expression is in fbm neurons just entering rhombomere 6.
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Specimen
6D':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 11.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
6D'
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Expression is in migrating fbm neurons. Expression in this mutant is the same as in wild-type.
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| TS18: rhombomere 5 |
Present |
Regionally restricted |
6D'
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Expression is in migrating fbm neurons. Expression in this mutant is the same as in wild-type.
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| TS18: rhombomere 6 |
Present |
Regionally restricted |
6D'
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Expression is in fbm neurons just entering rhombomere 6. Expression in this mutant is the same as in wild-type.
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Specimen
7B:
embryonic day 12.5
(more )
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
7B
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Expression is in facial bm neurons located in rhombomere 4,5 and 6.
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Specimen
7B':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
7B'
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In this mutant expression is in a subpopulation of fbm neurons abnormally migrating in rhombomere 5 as well as some fbm neurons normally migrating in rhombomere 6. Expression in rhombomere 4 is stronger than in wild-type embryos.
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Analysis Tools