Probe:	Pax6 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1A	Expression is detected in the dorsal neural tube and the dorsal prosencephalon including prosomeres 1-3, cerebral vesicle and optic vesicle but is absent from the roof plate.
TS15: neural tube	Present	Regionally restricted	1A	Expression is detected in the dorsal neural tube and the dorsal prosencephalon including prosomeres 1-3, cerebral vesicle and optic vesicle but is absent from the roof plate.
TS15: telencephalon	Present	Regionally restricted	1A	Expression is detected in the dorsal neural tube and the dorsal prosencephalon including prosomeres 1-3, cerebral vesicle and optic vesicle but is absent from the roof plate.
TS15: future midbrain	Absent		1A
TS15: rhombomere 1	Absent		1A
TS15: rhombomere 2	Present		1A
TS15: rhombomere 3	Present		1A
TS15: rhombomere 4	Present		1A
TS15: rhombomere 5	Present		1A
TS15: rhombomere 6	Present		1A
TS15: rhombomere 7	Present		1A
TS15: rhombomere 8	Present		1A

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	1D	Expression is detected in prosomeres 3 and 1 but only in ventral prosomere 2, ventral mesencephalon (increased over E9.5) and rhombomere 1.
TS17: midbrain	Present	Regionally restricted	1D	Expression is detected in prosomeres 3 and 1 but only in ventral prosomere 2, ventral mesencephalon (increased over E9.5) and rhombomere 1.
TS17: rhombomere 1	Present	Regionally restricted	1D	Expression is detected in prosomeres 3 and 1 but only in ventral prosomere 2, ventral mesencephalon (increased over E9.5) and rhombomere 1.
TS17: telencephalon	Present	Regionally restricted	1D	Expression is detected in prosomeres 3 and 1 but only in ventral prosomere 2, ventral mesencephalon (increased over E9.5) and rhombomere 1.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Absent		2B , 2A	Authors report that the mesencephalon is unlabeled.
TS17: diencephalon	Strong	Regionally restricted	2A , 2B	Heavy label is clustered in seperate dorsal and ventral patches of caudal prosomere 1.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2D , 2C	Expression is detected in ventral prosomere 1.

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Strong	Regionally restricted	3A	Heavy expression is detected caudal to the optic stalk and light expression is detected ventral to the optic stalk.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	5A	Expression is detected in prosomeres 1 and 3, dorsal prosomer 2, ventral mesencephalon and ventral rhombencephalon.
TS17: hindbrain	Present	Regionally restricted	5A	Expression is detected in prosomeres 1 and 3, dorsal prosomer 2, ventral mesencephalon and ventral rhombencephalon.
TS17: midbrain	Present	Regionally restricted	5A	Expression is detected in prosomeres 1 and 3, dorsal prosomer 2, ventral mesencephalon and ventral rhombencephalon.
TS17: telencephalon	Present	Regionally restricted	5A	Expression is detected in prosomeres 1 and 3, dorsal prosomer 2, ventral mesencephalon and ventral rhombencephalon.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	5B	Expression is detected in prosomere 3, a thin strip adjacent to midline in dorsal prosomer 2, ventral mesencephalon and rhombencephalon.
TS17: hindbrain	Present	Regionally restricted	5B	Expression is detected in prosomere 3, a thin strip adjacent to midline in dorsal prosomer 2, ventral mesencephalon and rhombencephalon.
TS17: midbrain	Present	Regionally restricted	5B	Expression is detected in prosomere 3, a thin strip adjacent to midline in dorsal prosomer 2, ventral mesencephalon and rhombencephalon.