Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:64021
Lin X, et al., Disruption of gastrulation and heparan sulfate biosynthesis in EXT1-deficient mice. Dev Biol. 2000 Aug 15;224(2):299-311
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1889629
|
|
Gene symbol:
|
T
|
|
Gene name:
|
brachyury, T-box transcription factor T
|
|
Probe:
|
T cDNA9
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
2. C.:
embryonic day 7.5
(more  )
Specimen
2. C.:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2. D.:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Ext1tm1Dews/Ext1tm1Dews
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
E6.5 WT:
embryonic day 6.5
(more )
Specimen
E6.5 WT:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive streak |
Very strong |
Regionally restricted |
|
Expression is intense in a small cluster of cells in the primitive streak.
|
Specimen
E6.5 -/-:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Ext1tm1Dews/Ext1tm1Dews
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools
