Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:60091 Moon AM, et al., Normal limb development in conditional mutants of Fgf4. Development. 2000 Mar;127(5):989-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:1353485
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe18
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5A F4^+/+ top ©

Specimen 5A F4+/+ top: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	5A F4^+/+ top	Expression is in the zone of polarizing activity.

Specimen 5A F4+/+ bottom: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	5A F4^+/+ bottom	Expression is in the zone of polarizing activity.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	5A F4^+/+ bottom	Expression is in the zone of polarizing activity.

5A F4^AP/N top ©

Specimen 5A F4^AP/N top: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	5A F4^AP/N top	As in wild type, expression is in the zone of polarizing activity.

Specimen 5A F4^AP/N bottom: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	5A F4^AP/N bottom	As in wild type, expression is in the zone of polarizing activity.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	5A F4^AP/N bottom	As in wild type, expression is in the zone of polarizing activity.

Specimen 5A F4^AP/N;T top: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	5A F4^AP/N;T top	As in wild type, expression is in the zone of polarizing activity.

Specimen 5A F4^AP/N;T bottom: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	5A F4^AP/N;T bottom	As in wild type, expression is in the zone of polarizing activity.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	5A F4^AP/N;T bottom	As in wild type, expression is in the zone of polarizing activity.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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