Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55208
Mechta-Grigoriou F, et al., Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain. Development. 2000 Jan;127(1):119-28
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1350576
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Gene symbol:
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Nab2
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Gene name:
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Ngfi-A binding protein 2
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Probe:
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Nab2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1N:
embryonic day 8.0
(more  )
Note: 1-somite stage.
Specimen
1N:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
1N
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Expression is in the presumptive prorhombomere 3.
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Specimen
1O:
embryonic day 8.0
(more )
Note: 4-somite stage.
Specimen
1O:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage.
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Specimen
1P:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
1P:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
1P
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Expression is in prorhombomeres 3 and 5.
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Specimen
1Q:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
1Q:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12-somite stage.
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Specimen
1R:
embryonic day 9.0
(more )
Note: 16-somite stage.
Specimen
1R:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: rhombomere 3 |
Weak |
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1R
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A weak level was chosen to indicate a progeressive down-regulation from the 12-somite stage.
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| TS14: rhombomere 5 |
Weak |
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1R
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A weak level was chosen to indicate a progeressive down-regulation from the 12-somite stage.
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Specimen
1S:
embryonic day 9.5
(more )
Note: 20-somite stage.
Specimen
1S:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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20-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 3 |
Weak |
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1S
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A weak level was chosen to indicate a progeressive down-regulation from the 12-somite stage.
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| TS15: rhombomere 5 |
Weak |
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1S
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A weak level was chosen to indicate a progeressive down-regulation from the 12-somite stage.
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Specimen
1T:
embryonic day 8.5; Egr2tm1Pch/Egr2 +
(more )
Note: 10-somite stage. Also stained for an Egr2 reporter (Krox20; assay MGI:7484071).
Specimen
1T:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10-somite stage. Also stained for an Egr2 reporter (Krox20; assay MGI:7484071).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: rhombomere 3 |
Present |
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1T
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Nab2 expression is contained within the Egr2 expression domain.
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| TS13: rhombomere 5 |
Present |
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1T
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Nab2 expression is contained within the Egr2 expression domain.
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Specimen
2D:
embryonic day 8.0
(more )
Note: 4 to 6-somite stage.
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4 to 6-somite stage.
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Specimen
2E:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 4 to 6-somite stage.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4 to 6-somite stage.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4 to 6 somites.
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Specimen
2G:
embryonic day 8.5
(more )
Note: 10 to 12-somite stage.
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 to 12-somite stage.
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Specimen
2H:
embryonic day 8.5; Egr2tm1Pch/Egr2 +
(more )
Note: 10 to 12 somite stage.
Specimen
2H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 to 12 somite stage.
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 to 12 somite stage.
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Analysis Tools