Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:55208
Mechta-Grigoriou F, et al., Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain. Development. 2000 Jan;127(1):119-28
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1350575
|
Gene symbol:
|
Nab1
|
Gene name:
|
Ngfi-A binding protein 1
|
Probe:
|
Nab1 cDNA
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1G:
embryonic day 8.0
(more )
Note: 0-somite stage.
Specimen
1G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
0-somite stage.
|
Specimen
1H:
embryonic day 8.0
(more )
Note: 4-somite stage.
Specimen
1H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4-somite stage.
|
Specimen
1I:
embryonic day 8.5
(more )
Note: 8-somite stage.
Specimen
1I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: rhombomere 3 |
Weak |
|
1I
|
The expression level was assigned by a GXD editor based on compasisons in the text.
|
TS13: rhombomere 5 |
Moderate |
Graded |
1I
|
There is higher expression at the boundaries. The expression level was assigned by a GXD editor based on compasisons in the text.
|
Specimen
1J:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
1J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
12-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: rhombomere 3 |
Weak |
Uniform |
1J
|
The expression level was assigned by a GXD editor based on compasisons in the text.
|
TS13: rhombomere 5 |
Moderate |
Uniform |
1J
|
The expression level was assigned by a GXD editor based on compasisons in the text.
|
Specimen
1K:
embryonic day 9.0
(more )
Note: 16-somite stage.
Specimen
1K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
16-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: rhombomere 3 |
Weak |
Uniform |
1K
|
The level of expression is getting progressively lower starting from the ventral part of the rhombomere.
|
TS14: rhombomere 5 |
Weak |
Uniform |
1K
|
The level of expression is getting progressively lower starting from the ventral part of the rhombomere.
|
Specimen
1L:
embryonic day 9.5
(more )
Note: 22-somite stage.
Specimen
1L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
22-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: rhombomere 3 |
Present |
Uniform |
1L
|
The level of expression is getting progressively lower starting from the ventral part of the rhombomere.
|
TS15: rhombomere 5 |
Present |
Uniform |
1L
|
The level of expression is getting progressively lower starting from the ventral part of the rhombomere.
|
Specimen
1M:
embryonic day 9.0; Egr2tm1Pch/Egr2 +
(more )
Note: 14-somite stage. Also stained for an Egr2 reporter (Krox20; assay MGI:7484071).
Specimen
1M:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
14-somite stage. Also stained for an Egr2 reporter (Krox20; assay MGI:7484071).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: rhombomere 3 |
Present |
|
1M
|
The expression is contained entirely within the Egr2 expression domain.
|
TS14: rhombomere 5 |
Present |
|
1M
|
The expression is contained entirely within the Egr2 expression domain.
|
Specimen
2A:
embryonic day 8.5
(more )
Note: 8 to 10-somite stage.
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8 to 10-somite stage.
|
Specimen
2B:
embryonic day 8.5; Egr2tm1Pch/Egr2 +
(more )
Note: 8 to 10-somite stage.
Specimen
2B:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8 to 10-somite stage.
|
Specimen
2C:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8 to 10 somite stage.
|
|