About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:58413 Wasiak S, et al., Retinoic acid affects left-right patterning. Dev Biol. 1999 Nov 15;215(2):332-42
Assay type: RNA in situ
MGI Accession ID: MGI:1350410
Gene symbol: Nodal
Gene name: nodal growth differentiation factor
Probe: Nodal cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Embryos were fixed in 4% paraformaldehyde/2% gluteraldehyde.
Results
Specimen not shown: embryonic day 8.0 (more )
Note: 1-3 somites. This embryo's mother was given corn oil by oral gavage.
Structure Level Pattern Image Note
TS12: primitive streak Present Regionally restricted At 1-2 somites, expression is restricted to the ventral lateral regions surrounding the node. At 3 somites, it becomes slightly more pronounced to the left.
Specimen 3A: embryonic day 8.0 (more )
Note: 4-6 somites. This embryo's mother was given corn oil by oral gavage.
Structure Level Pattern Image Note
TS12: lateral plate mesoderm Present Regionally restricted 3A The transcripts spread anteriorly. The rostral limit reaches the level of the developing heart by 5-6 somites.
TS12: primitive streak Present Regionally restricted 3A The transcripts spread anteriorly. The rostral limit reaches the level of the developing heart by 5-6 somites.
Specimen not shown: embryonic day 8.0 (more )
Note: 7 somites. This embryo's mother was given corn oil by oral gavage.
Structure Level Pattern Image Note
TS12: lateral plate mesoderm Absent
TS12: primitive streak Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory