Probe:	mRAR-gamma
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted	1B , 1A	Expression is detected in the precartilaginous and cartilaginous skeletal elements.
TS21: genital tubercle	Present		1B , 1A

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		2B

Structure	Level	Pattern	Image	Note
TS12: head somite	Absent		2C
TS12: trunk somite	Absent		2C
TS12: embryo	Strong	Regionally restricted	2C	Expression is restricted to the caudal portion of the embryo.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Regionally restricted	2D	Expression is detected in presomitic mesoderm, neurectoderm of the open neural folds and the "overlying" ectoderm.
TS12: neural ectoderm	Present	Regionally restricted	2D	Expression is detected in presomitic mesoderm, neurectoderm of the open neural folds and the "overlying" ectoderm.
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2D	Expression is detected in presomitic mesoderm, neurectoderm of the open neural folds and the "overlying" ectoderm.
TS12: embryo endoderm	Present		2D
TS12: lateral plate mesoderm	Present		2D

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present	Regionally restricted	3A	Uniform expression is detected in the head mesenchyme except in the frontonasal mesenchyme and the mandibular arch where the expression is stronger.
TS13: 1st branchial arch	Strong		3A

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Strong	Regionally restricted	3B	Strong expression was detected in the frontonasal mesenchyme.
TS13: embryo mesenchyme	Present	Regionally restricted	3B	Expression is detected in the posterior of the embryo in the neural groove and the underlying undifferentiated mesenchyme.
TS13: future spinal cord neural fold	Present	Regionally restricted	3B	Expression is detected in the posterior of the embryo in the neural groove and the underlying undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Present	Regionally restricted	3C	Expression was detected in the frontonasal mesenchyme.
TS14: 1st branchial arch mesenchyme	Present	Uniform	3C
TS14: 2nd branchial arch mesenchyme	Present	Uniform	3C
TS14: embryo mesenchyme	Weak	Regionally restricted	3C	Weak expression was detected in the non-arch mesenchyme.
TS14: mesenchyme derived from lateral plate	Present		3C

Structure	Level	Pattern	Image	Note
TS14: forelimb bud	Present	Uniform	3D

Structure	Level	Pattern	Image	Note
TS17: frontal process mesenchyme	Present		3E
TS17: latero-nasal process mesenchyme	Present		3E
TS17: tail sclerotome	Present		3E
TS17: branchial arch	Present	Regionally restricted	3E	Expression was detected in the pharyngeal arch mesenchyme and the lateral mesoderm.
TS17: embryo mesenchyme	Present	Regionally restricted	3E	Expression was detected in the pharyngeal arch mesenchyme and the lateral mesoderm.
TS17: forelimb bud	Present	Regionally restricted	3E	Expression is homogeneous in the limb buds except it is absent from the apical ectodermal ridges.
TS17: hindlimb bud	Present	Regionally restricted	3E	Expression is homogeneous in the limb buds except it is absent from the apical ectodermal ridges.
TS17: embryo ectoderm	Absent		3E
TS17: forelimb bud apical ectodermal ridge	Absent		3E
TS17: hindlimb bud apical ectodermal ridge	Absent		3E

Structure	Level	Pattern	Image	Note
TS17: frontal process mesenchyme	Present		3F
TS17: head mesenchyme	Present		3F
TS17: latero-nasal process mesenchyme	Present		3F
TS17: eye	Absent		3F
TS17: forebrain	Absent		3F
TS17: nasal pit	Absent		3F

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present
TS16: hindlimb bud	Present

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present
TS19: trunk somite	Present

Structure	Level	Pattern	Image	Note
TS20: otic capsule	Strong	Regionally restricted	4A	Expression was strong in sclerotome derived precartilage and precartilaginous condensations.
TS20: vertebral cartilage condensation	Strong	Regionally restricted	4A	Expression was strong in sclerotome derived precartilage and precartilaginous condensations.
TS20: vertebral pre-cartilage condensation	Strong	Regionally restricted	4A	Expression was strong in sclerotome derived precartilage and precartilaginous condensations.
TS20: frontal process mesenchyme	Present	Regionally restricted	4A	Expression detected in frontonasal and pharyngeal arch mesenchyme.
TS20: head mesenchyme	Present	Regionally restricted	4A	Expression detected in frontonasal and pharyngeal arch mesenchyme.
TS20: latero-nasal process mesenchyme	Present	Regionally restricted	4A	Expression detected in frontonasal and pharyngeal arch mesenchyme.
TS20: mandible primordium	Present	Regionally restricted	4A	Expression detected in frontonasal and pharyngeal arch mesenchyme.
TS20: inner ear	Present	Regionally restricted	4A	Although there is expression present elsewhere in the inner ear, there is no expression seen in the otocyst-derived tissue.

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Strong		4B	Expression is strong in sclerotome derived precartilage.
TS20: left lung lobar bronchus mesenchyme	Present	Regionally restricted	4B	Expression is detected in the undifferentiated mesenchyme of the bronchi.
TS20: right lung mesenchyme	Present	Regionally restricted	4B	Expression is detected in the undifferentiated mesenchyme of the bronchi.
TS20: forelimb pre-cartilage condensation	Present		4B
TS20: rib pre-cartilage condensation	Present		4B
TS20: brain	Absent		4B
TS20: spinal cord	Absent		4B
TS20: hand	Present	Uniform	4B

Structure	Level	Pattern	Image	Note
TS20: cervical vertebral cartilage condensation	Strong		5A	Expression was strong in the sclerotome derived precartilage (prevertebrae). Expression was uniformly distributed in the precartilaginous blastema.

Structure	Level	Pattern	Image	Note
TS22: cervical vertebral cartilage condensation	Present		5B
TS22: cervical vertebra	Absent		5B

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Present	Uniform	5C	Expression was uniformly distributed in the precartilaginous blastema.
TS20: shoulder mesenchyme	Strong		5C	Expression is restricted to the pre-cartilage condensations of the shoulder girdle.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present		5D
TS22: scapula cartilage condensation	Present		5D

Structure	Level	Pattern	Image	Note
TS20: foot	Strong	Regionally restricted	5E	Expression is in most of the mesenchyme with higher levels in the blastema.

Structure	Level	Pattern	Image	Note
TS22: foot cartilage condensation	Present		5F

Structure	Level	Pattern	Image	Note
TS21: foot pre-cartilage condensation	Present		6A Center

Structure	Level	Pattern	Image	Note
TS21: otic capsule	Present		6B Center

Structure	Level	Pattern	Image	Note
TS22: cervical vertebral cartilage condensation	Present		7A
TS22: laryngeal cartilage	Present		7A
TS22: oral epithelium	Present		7A
TS22: sternebra pre-cartilage condensation	Present		7A
TS22: thoracic vertebral cartilage condensation	Present		7A
TS22: lung	Present	Regionally restricted	7A	Expression was detected in the cartilage of the trachea and the surrounding the bronchioles.
TS22: trachea	Present	Regionally restricted	7A	Expression was detected in the cartilage of the trachea and the surrounding the bronchioles.

Structure	Level	Pattern	Image	Note
TS22: esophagus	Present		7B
TS22: stomach	Present	Regionally restricted	7B	Expression was detected in the left wall of the stomach.
TS22: stomach glandular region epithelium	Absent		7B

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	7C	Expression was detected at the root of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS21: tooth	Present