Probe:	Hoxb13 probe-2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: tail	Present	Regionally restricted	8A	Expression was restricted to the tailbud.

Structure	Level	Pattern	Image	Note
TS20: tail	Present	Regionally restricted	8B	Expression was restricted to the tailbud.

Structure	Level	Pattern	Image	Note
TS20: hindgut	Present	Regionally restricted	8C	Expression was confined to the lining of the hindgut and did not reach the full caudal extent of the structure, the anal canal.
TS20: anal region	Absent		8C
TS20: urogenital sinus	Present		8C
TS20: tail	Present	Regionally restricted	8C	Expression was restricted to the tailbud.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	8D	Expression in the spinal cord was restricted to the caudal extent of the mantle layer, with higher levels found in the ventral grey horn.

Structure	Level	Pattern	Image	Note
TS20: tail	Strong	Regionally restricted	8E	Expression was strong in the tailbud.
TS20: spinal cord	Present	Regionally restricted	8E	Expression was detected in the caudal region of the spinal cord.