Gene Expression Data
Assay Details
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Assay
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Reference:
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J:53903
Theil T, et al., Gli3 is required for Emx gene expression during dorsal telencephalon development. Development. 1999 Aug;126(16):3561-71
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340420
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense RNA
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Assay notes:
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Xt<J>/Xt<J> embryos that were classified as exencephalic (based on overall brain morphology) were excluded from this study.
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Results
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Specimen
not shown:
embryonic day 8.5; Gli3Xt-J/ Gli3Xt-J
(more  )
Note: 10-12 somites.
Specimen
not shown:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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10-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
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Authors report no change in expression before the 10-12 somite stage.
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
5A
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Transcripts are confined to the commissural plate.
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Specimen
5B:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
5B
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Expression starts to extend into more caudal regions of the telencephalic roof plate in the mutant.
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Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
5C
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Transcripts are confined to the commissural plate.
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Specimen
5D:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
5D
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In contrast to the wild-type, expression covers the complete telencephalic roof.
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Analysis Tools
