Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339651
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
1D:
embryonic day 9.5
(more  )
Note: Limb-bud stage (stage 1).
Specimen
1D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1D
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Expression is in the distal half of the ventral limb bud.
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Specimen
1F:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3).
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3).
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Specimen
2H:
embryonic day 10.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Limb-bud stage (stage 3/4).
Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4).
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Specimen
3K:
embryonic day 11.5-12.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 7/8). Double labeled: purple - Fgf8; pink - En1 reporter (assay MGI:5318340).
Specimen
3K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 7/8). Double labeled: purple - Fgf8; pink - En1 reporter (assay MGI:5318340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot |
Present |
Regionally restricted |
3K
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In mutants there is ectopic expression in foci of ventral ectoderm, while expression is down-regulated in the primary apical ectodermal ridge.
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| TS20: hand |
Present |
Regionally restricted |
3K
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In mutants there is ectopic expression in foci of ventral ectoderm, while expression is down-regulated in the primary apical ectodermal ridge.
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Specimen
3L:
embryonic day 11.5-12.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 7/8). Double labeled: purple - Fgf8; pink - En1 reporter (assay MGI:5318340).
Specimen
3L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 7/8). Double labeled: purple - Fgf8; pink - En1 reporter (assay MGI:5318340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot |
Present |
Regionally restricted |
3L
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In mutants there is ectopic expression in foci of ventral ectoderm, while expression is down-regulated in the primary apical ectodermal ridge.
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| TS20: hand |
Present |
Regionally restricted |
3L
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In mutants there is ectopic expression in foci of ventral ectoderm, while expression is down-regulated in the primary apical ectodermal ridge.
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Specimen
5A:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 4).
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 4).
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Specimen
5B:
embryonic day 10.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 4).
Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 4).
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Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 4).
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 4).
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5-8).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot |
Present |
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No mutants developed a second ventral rim or appeared bifurcated.
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| TS20: hand |
Present |
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No mutants developed a second ventral rim or appeared bifurcated.
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