Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5318340
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
2I:
embryonic day 10.5; En1tm2Alj/En1 +
(more  )
Note: Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
Specimen
2I:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
2I
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Expression is throughout the ventral limb ectoderm, extending to the distal tip.
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Specimen
2J:
embryonic day 10.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
Specimen
2J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
2J
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Expression is throughout the ventral limb ectoderm, extending to the distal tip.
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Specimen
3C:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Limb-bud stage (stage 5/6). There are discrepancies between the figure and the figure legend as to the genotype of the embryo.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6). There are discrepancies between the figure and the figure legend as to the genotype of the embryo.
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Specimen
3D:
embryonic day 11.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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Specimen
3I:
embryonic day 11.5-12.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
Specimen
3I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). Double labeled: pink - En1 reporter; purple - Lmx1b (assay MGI:1339623).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
3I
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Expressed in the apical ectodermal ridge.
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Specimen
3K:
embryonic day 11.5-12.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 7/8). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
Specimen
3K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 7/8). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
3K
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Expressed in the apical ectodermal ridge.
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Specimen
3L:
embryonic day 11.5-12.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 7/8). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
Specimen
3L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 7/8). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
3L
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Expressed in the apical ectodermal ridge.
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Specimen
4A:
embryonic day 12.5-13.5; En1tm2Alj/En1 +
(more )
Note: Limb-bud stage (stage 8/9).
Specimen
4A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 8/9).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Present |
Regionally restricted |
4A
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Expressed in a remnant ridge along the dorsal-ventral boundary.
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Specimen
4B:
embryonic day 12.5-13.5; En1tm1Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 8/9).
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 8/9).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Present |
Regionally restricted |
4B
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In some mutants, expressions are in the dorsal and ventral rims, which are very closely apposed, forming a slightly wider linear ridge than the wild-type.
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Specimen
4C:
embryonic day 12.5-13.5; En1tm1Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 8/9).
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 8/9).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Present |
Regionally restricted |
4C
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In some mutants, expressions are in the dorsal and ventral rims, which are closer to each other than earlier developmental stages.
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Specimen
4D:
embryonic day 12.5-13.5; En1tm1Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 8/9).
Specimen
4D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 8/9).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Present |
Regionally restricted |
4D
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In some mutants, expressions are in the dorsal and ventral rims. The ventral rims are not yet tightly apposed to the dorsal rims, form a small deflection or bow in the otherwise linear contour of the ventral rim.
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Specimen
4E/F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 13.5-15.0
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Age Note:
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Ages are not specified, assigned according to limb-bud stage.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb digit |
Present |
Regionally restricted |
4E
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4F
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Expressed in secondary linear ridges which join the apical ectodermal ridge at a 90 degree angle.
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Specimen
4G/H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm1Alj/En1tm2Alj
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Ages are not specified, assigned according to limb-bud stage.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb digit |
Present |
Regionally restricted |
4G
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4H
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Secondary ridges which developed more proximally overlie differentiating ectopic digits. They appear to be discontinuous with the primary ridge. If viewed from the side, some are found to be linked to the primary ridge.
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Specimen
4I:
embryonic day 13.5-14.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 10). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 13.5-14.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 10). Double labeled: pink - En1 reporter; purple - Fgf8 (assay MGI:1339651).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: limb |
Present |
Regionally restricted |
4I
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Expressed in the dorsal and ventral rims of the remnant apical ectodermal ridge.
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Specimen
4K:
embryonic day 15.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 12).
Specimen
4K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 15.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 12).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: limb |
Present |
Regionally restricted |
4K
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Expressed in an early ventral digit.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 4).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud apical ectodermal ridge |
Present |
Regionally restricted |
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Expression in the apical ectodermal ridge seems to be slightly wider than in wild-type embryos. The dorsal, rather than the ventral, portion was primarily expanded.
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