Antibody:	ANTI-SLF
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: tail dermomyotome	Present	Regionally restricted	3A	Expression is in the dermatome in the trunk.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present	Regionally restricted	3B	Expression is in the trunk. The author describes this expression as in the pronephros. Mesonephros was chosen to be consistent with the anatomical dictionary at this stage.

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Present	Regionally restricted	3C	Expression is in the trunk. The author uses the term genital ridge.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present	Regionally restricted	3D	Expression is along the entire embryonic axis.

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Present		3E	Increased expression is associated with the neuronal fiber tracks crossing the floorplate.

Structure	Level	Pattern	Image	Note
TS17: tail dermomyotome	Weak	Regionally restricted	3F	Expression is restricted to the luminal aspect.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Weak	Regionally restricted	3G	Expression is restricted to the luminal aspect. The authors use the term pronephros. Mesonephros was chosen to be consistent with the anatomical dictionary at this stage.

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Weak	Regionally restricted	3H	The author uses the term genital ridge.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Weak	Regionally restricted	3I	In mutant embryos, expression is found only in the luminal aspect.

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Weak	Regionally restricted	3J	Absence of expression associated with the neuronal fiber tracks crossing the floorplate.

Structure	Level	Pattern	Image	Note
TS19: gonad	Weak			Expression in mutants is reduced compared to wild-type embryos.
TS19: midbrain floor plate	Weak			Expression in mutants is reduced compared to wild-type embryos.
TS19: neural tube floor plate	Weak			Expression in mutants is reduced compared to wild-type embryos.

Structure	Level	Pattern	Image	Note
TS21: tail mesenchyme	Absent			In mutant embryos, expression is not detected in the dermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Strong		6A	Expression levels have increased from 10.5dpc embryos.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present	Regionally restricted	6B	Expression is localized to the basal aspect of the floorplate.

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present	Regionally restricted	6C	Expression is in basolateral area of epidermal keratinocytes.

Structure	Level	Pattern	Image	Note
TS20: diencephalon floor plate	Present	Regionally restricted	6E	The location along the floorplate is not specified. Expression is basolateral.
TS20: medulla oblongata floor plate	Present	Regionally restricted	6E	The location along the floorplate is not specified. Expression is basolateral.
TS20: metencephalon floor plate	Present	Regionally restricted	6E	The location along the floorplate is not specified. Expression is basolateral.
TS20: midbrain floor plate	Present	Regionally restricted	6E	The location along the floorplate is not specified. Expression is basolateral.
TS20: spinal cord floor plate	Present	Regionally restricted	6E	The location along the floorplate is not specified. Expression is basolateral.

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Regionally restricted	6G	Expression is in condensed mesenchyme surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Present	Regionally restricted		Expression is basolateral.