Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39344
Yoshikawa Y, et al., Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse. Dev Biol. 1997 Mar 15;183(2):234-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1338373
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5G,H:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Wnt3atm1Amc/Wnt3atm1Amc
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
5H
,
5G
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In Wnt3a-mutant embryos, expression is in the ectopic neural tube where the tube contacts the notochord in the anterior region (5G) but not in the posterior region lacking the notochord (5H).
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Analysis Tools
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