Gene Expression Data
Assay Details
Assay
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Reference:
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J:29276
Acampora D, et al., Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-90
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336361
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2d:
embryonic day 5.75
(more )
Specimen
2d:
(close )
Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 5.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2i:
embryonic day 6.5
(more )
Specimen
2i:
(close )
Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
2i
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Expression is in the external layer with a progressive anteriorization.
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TS9: embryo ectoderm |
Present |
Regionally restricted |
2i
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There is a progressive anteriorization of expression.
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Specimen
2n:
embryonic day 7.75
(more )
Specimen
2n:
(close )
Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
|
embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo ectoderm |
Present |
Regionally restricted |
2n
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Expression is restricted to the anterior one third of the embryo.
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TS11: embryo endoderm |
Present |
Regionally restricted |
2n
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Expression is restricted to the anterior one third of the embryo.
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TS11: embryo mesoderm |
Present |
Regionally restricted |
2n
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Expression is restricted to the anterior one third of the embryo.
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