Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:26913
Dickson MC, et al., Defective haematopoiesis and vasculogenesis in transforming growth factor-beta 1 knock out mice. Development. 1995 Jun;121(6):1845-54
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1334715
|
|
Gene symbol:
|
Kdr
|
|
Gene name:
|
kinase insert domain protein receptor
|
|
Probe:
|
flk-1 probe4
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The fixation for this assay was 4% paraformaldehyde/0.2% gluteraldehyde.
|
|
|
Results
|
Specimen
3A:
embryonic day 8.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * NIH/Ola
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: extraembryonic component |
Ambiguous |
Regionally restricted |
3A
|
Expression is in the allantois and the endothelial cells of the yolk sac. 12/17 embryos were scored as having high levels of expression in the yolk sac endothelium.
|
| TS12: embryo |
Strong |
Regionally restricted |
3A
|
Expression is in endothelial cells.
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * NIH/Ola
|
|
Mutant Allele(s):
|
Tgfb1tm1N/Tgfb1tm1N
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: extraembryonic component |
Ambiguous |
Regionally restricted |
3B
|
4/13 embryos showed high expression in the yolk sac endothelial cells.
|
| TS12: embryo |
Strong |
Regionally restricted |
3B
|
Expression is in endothelial cells.
|
|
Analysis Tools
