Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:19212
Wurst W, et al., Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development. 1994 Jul;120(7):2065-75
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1334707
|
|
Gene symbol:
|
En2
|
|
Gene name:
|
engrailed 2
|
|
Probe:
|
pBSEn-2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4F:
embryonic day 8.5
(more  )
Note: Around the 10-somite stage.
Specimen
4F:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Around the 10-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
4F
|
Expression is in a wedge across the mid-hindbrain junction.
|
| TS14: future midbrain |
Present |
Regionally restricted |
4F
|
Expression is in a wedge across the mid-hindbrain junction.
|
Specimen
4G:
embryonic day 8.5; En1tm1Alj/ En1tm1Alj
(more )
Note: Around the 10-somite stage.
Specimen
4G:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
|
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Around the 10-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
4G
|
In mutants, only a dorsal patch of expression is present.
|
| TS13: future midbrain |
Present |
Regionally restricted |
4G
|
In mutants, only a dorsal patch of expression is present.
|
|
Analysis Tools
