Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43311
Peichel CL, et al., The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning. Development. 1997 Sep;124(18):3481-92
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333608
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Gene symbol:
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Hoxd11
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Gene name:
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homeobox D11
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Probe:
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Hoxd11 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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See the Materials and Methods for details of the possible strain backgrounds.
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Results
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Specimen
2C +/+ upper:
embryonic day 11.0
(more  )
Specimen
2C +/+ upper:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2C +/+ upper
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Expression is strong in the prospective zeugopod (radius and ulna) and weak in the prospective autopod (digits).
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Specimen
2C +/+ lower:
embryonic day 11.0
(more )
Specimen
2C +/+ lower:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
2C +/+ lower
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Expression is strong in the prospective zeugopod (radius and ulna) and weak in the prospective autopod (digits).
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Specimen
2C Ul/+ upper:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2C Ul/+ upper:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2C Ul/+ upper
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There is a reduction in proximal domain of expression in the forelimbs.
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Specimen
2C Ul/+ lower:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2C Ul/+ lower:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C Ul/Ul upper:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2C Ul/Ul upper:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2C Ul/Ul upper
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There is a reduction in expression in the proximal domain of both forelimbs and hindlimbs. The distal domain of expression is also reduced in forelimbs.
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Specimen
2C Ul/Ul lower:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2C Ul/Ul lower:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
2C Ul/Ul lower
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There is a reduction of expression in both the proximal and distal domains.
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Specimen
5A +/+:
embryonic day 11.0
(more )
Specimen
5A +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A Ul/+:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
5A Ul/+:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/Lnpk+
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A Ul/Ul:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
5A Ul/Ul:
(close )
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Genetic Background:
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either: (involves: FVB/N) or (involves: MOLF/Ei)
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Mutant Allele(s):
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LnpkUl/LnpkUl
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools