Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44237
Goh KL, et al., Mesodermal defects and cranial neural crest apoptosis in alpha5 integrin-null embryos. Development. 1997 Nov;124(21):4309-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331628
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T cDNA9
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Assay notes:
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The fixative for these embryos is 4% paraformaldehyde/ 0.1% gluteraldehyde.
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Results
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Specimen
7A-/-, 7B:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Itga5tm1Hyn/Itga5tm1Hyn
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7A wt:
embryonic day 8.5
(more  )
Specimen
7A wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
8A:
embryonic day 8.5; Itga5tm1Hyn/ Itga5tm1Hyn
(more )
Note: This section is from the whole mount shown in Figure 7B.
Specimen
8A:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Itga5tm1Hyn/Itga5tm1Hyn
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This section is from the whole mount shown in Figure 7B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
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8A
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Expression was detected in mutant notochord as in wild type.
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Specimen
8B:
embryonic day 8.5; Itga5tm1Hyn/ Itga5tm1Hyn
(more )
Note: This section is from the whole mount shown in Fig. 7B and is serial to the section in Fig. 8A.
Specimen
8B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Itga5tm1Hyn/Itga5tm1Hyn
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This section is from the whole mount shown in Fig. 7B and is serial to the section in Fig. 8A.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Absent |
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8B
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There was a complete absence of the notochord structure.
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Specimen
8C:
embryonic day 8.5; Itga5tm1Hyn/ Itga5tm1Hyn
(more )
Note: This section is from the whole mount shown in Figure 7B.
Specimen
8C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Itga5tm1Hyn/Itga5tm1Hyn
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This section is from the whole mount shown in Figure 7B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Absent |
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8C
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The notochord was present in this section, but staining was absent.
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