Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331424
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Probe:
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En2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 10.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
3A
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Expression extends from the anterior midbrain to the rhombomere1/rhombomere2 boundary.
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| TS17: rhombomere 1 |
Present |
Regionally restricted |
3A
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Expression extends from the anterior midbrain to the rhombomere1/rhombomere2 boundary.
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| TS17: rhombomere 2 |
Present |
Regionally restricted |
3A
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Expression extends from the anterior midbrain to the rhombomere1/rhombomere2 boundary.
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Specimen
3B:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
3B
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The expression domain caudal to the isthmic constriction appears to be substantially reduced.
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| TS17: rhombomere 1 |
Present |
Regionally restricted |
3B
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The expression domain caudal to the isthmic constriction appears to be substantially reduced.
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| TS17: rhombomere 2 |
Present |
Regionally restricted |
3B
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The expression domain caudal to the isthmic constriction appears to be substantially reduced.
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Analysis Tools
