Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46225
Hayes C, et al., Sonic hedgehog is not required for polarising activity in the Doublefoot mutant mouse limb bud. Development. 1998 Feb;125(3):351-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1310054
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Results
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Specimen
4A:
embryonic day 10.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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C3H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4B:
embryonic day 10.5; Dbf/Dbf +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Uniform |
4B
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Ptc was expressed throughout the distal mesenchyme in a continuous posterior-to-anterior domain.
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Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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C3H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
5B:
embryonic day 10.5; Dbf/Dbf +
(more )
Specimen
5B:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
5B
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Ptc was expressed in a broad anteroposterior band that extends further anteriorly.
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Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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C3H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
5D:
embryonic day 11.5; Dbf/Dbf +
(more )
Specimen
5D:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Strong |
Regionally restricted |
5D
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Mutant limbs showed downregulation in all regions except for the anterior domain, where relatively high transcript levels remained.
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Specimen
not shown:
embryonic day 11.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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C3H
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Weak |
Regionally restricted |
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The transcript level was lower mid-distally, indicating early downregulation in this site.
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