Antibody:	anti-NF160 antibody (clone NN18) (N5264 or MAB5254)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	6A	Expression was detected in the mandibular branch of the trigeminal nerve and in the facial nerve.
TS17: peripheral nervous system	Present		6A

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	6B	As in the wild type embryo, expression was detected in the mandibular branch of the trigeminal nerve and in the facial nerve. However, there were abnormalities in nerve development in the mutant.
TS17: peripheral nervous system	Present		6B	Expression was detected as in wild type embryos.

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	6C	Expression was detected in the mandibular and maxillary branches of the trigeminal nerve and in the facial nerve (which was not shown).
TS19: glossopharyngeal nerve	Present		6C
TS19: peripheral nervous system	Present		6C

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	6D	As in the wild type embryo, expression was detected in the mandibular and maxillary branches of the trigeminal nerve and in the facial nerve (which was not shown). However, there were abnormalities in the nerve development in the mutant.
TS19: glossopharyngeal nerve	Present		6D	As in the wild type embryo, expression was detected in the glossopharyngeal nerve. However, there were abnormalities in the nerve development in the mutant.
TS19: peripheral nervous system	Present		6D	Expression was detected as in wild type embryos.