Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:43752
Xiang M, et al., Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development. 1998 Oct;125(20):3935-46
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1277990
|
|
Gene symbol:
|
Pou4f3
|
|
Gene name:
|
POU domain, class 4, transcription factor 3
|
|
Antibody:
|
anti-Brn-3c
|
|
Detection system:
|
Secondary antibody coupled to rhodamine
|
|
|
Results
|
Specimen
1K:
embryonic day 14.5
(more  )
Note: Double labeled: red - Pou4f3; green - BrdU.
Specimen
1K:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Pou4f3; green - BrdU.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inner ear vestibular component |
Present |
Regionally restricted |
1K
|
Expression was detected in hair cells of the lumenal layer as well as scattered cells in the supporting layer. The immunostaining does not overlap with BrdU staining, indicating that expression is in post-mitotic cells.
|
Specimen
Not Shown:
embryonic day 13.5
(more )
Note: Also labeled with BrdU.
Specimen
Not Shown:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Also labeled with BrdU.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear vestibular component |
Present |
Regionally restricted |
|
Expression was detected in hair cells of the lumenal layer as well as scattered cells in the supporting layer. The immunostaining does not overlap with BrdU staining, indicating that expression is in post-mitotic cells.
|
|
Analysis Tools
