Analysis Tools
immune system
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• RT-qPCR analysis shows upregulation of inflammatory genes Il6, Il23a, and Tbx21 in the colon suggesting a heightened basal inflammatory state
• mice with DSS-induced colitis exhibit multiple markers of increased inflammation, including decreased lamina propria cell numbers and increased percentages of IL-17a+ immune cells, IFN-gamma+ CD8+T cells and CD4+ T cells, and myeloid cells, and decreased percentages of TGF-beta immune cells
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• 6- to 8-week-old mice challenged with dextran sulfate sodium (DSS) to induce colitis exhibit increased susceptibility to colitis, with worsened pathology, greater colon shortening at day 6 of DSS treatment, and higher colitis scores than wild-type mice
• mice with DSS-induced colitis exhibit a 5-fold increase in Tbx21 expression in both conventional T (Tconv) cells and regulatory T (Treg) cells and a higher frequency of T-BET+ cells among lamina propria Tconv cells and Treg cells in colons
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• mice challenged with DSS show higher mortality, with approximately 60% of mice dying by day 6
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• ex vivo differentiation of CD4 T helper subsets show an increase in T-bet expression during Th1 and Th17 differentiation, along with reduced Gata3 expression under Th2 conditions, indicative of a skewing toward type 1 responses
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• at baseline, mice exhibit an increased frequency of peripheral effector CD4+ conventional T (Tconv) cells
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mortality/aging
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• mice challenged with DSS show higher mortality, with approximately 60% of mice dying by day 6
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growth/size/body
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• mice challenged with DSS exhibit greater weight loss than wild-type mice, with about 3-fold greater loss by day 6
• transfer of naive mutant CD4+ Tconv cells into lymphopenic recipients results in greater weight loss compared with wild-type Tconv cell recipients
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digestive/alimentary system
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• RT-qPCR analysis shows upregulation of inflammatory genes Il6, Il23a, and Tbx21 in the colon suggesting a heightened basal inflammatory state
• mice with DSS-induced colitis exhibit multiple markers of increased inflammation, including decreased lamina propria cell numbers and increased percentages of IL-17a+ immune cells, IFN-gamma+ CD8+T cells and CD4+ T cells, and myeloid cells, and decreased percentages of TGF-beta immune cells
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• 6- to 8-week-old mice challenged with dextran sulfate sodium (DSS) to induce colitis exhibit increased susceptibility to colitis, with worsened pathology, greater colon shortening at day 6 of DSS treatment, and higher colitis scores than wild-type mice
• mice with DSS-induced colitis exhibit a 5-fold increase in Tbx21 expression in both conventional T (Tconv) cells and regulatory T (Treg) cells and a higher frequency of T-BET+ cells among lamina propria Tconv cells and Treg cells in colons
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• mice challenged with DSS show higher mortality, with approximately 60% of mice dying by day 6
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hematopoietic system
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• ex vivo differentiation of CD4 T helper subsets show an increase in T-bet expression during Th1 and Th17 differentiation, along with reduced Gata3 expression under Th2 conditions, indicative of a skewing toward type 1 responses
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• at baseline, mice exhibit an increased frequency of peripheral effector CD4+ conventional T (Tconv) cells
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