Phenotypes associated with this allele

• Allele Symbol: Rnasek^em1Xlt
• Allele Name: endonuclease-mediated mutation 1, Xiao-Li Tian
• Allele ID: MGI:8401752
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rnasek^em1Xlt/Rnasek^em1Xlt	involves: C57BL/6J	MGI:8401753

Genotype
MGI:8401753

hm1

• Allelic Composition: Rnasek^em1Xlt/Rnasek^em1Xlt
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:386262 )

• no viable embryos are recovered at E6.5, E7.5, or E8.5

• examination of isolated decidual tissues indicate that embryos successfully implant but fail to progress beyond the egg cylinder stage

embryo

increased embryonic epiblast cell apoptosis ( J:386262 )

• elevated levels of caspase-3 in embryos at E5.0

decreased embryonic epiblast cell proliferation ( J:386262 )

• decreased proliferation coinciding with an increase in gamma H2AX foci indicating premature senescence

embryonic growth arrest ( J:386262 )

• fail to progress beyond the egg cylinder stage

decreased embryo size ( J:386262 )

• at E5.5

• at E5.0 the total number of cells in all three lineages (epiblast, visceral endoderm, and extraembryonic ectoderm) is reduced

cellular

impaired autophagy ( J:386262 )

• blockade in autophagic flux

• following infection with a reporter adenovirus embryos show an increase in autophagosomes

increased embryonic epiblast cell apoptosis ( J:386262 )

• elevated levels of caspase-3 in embryos at E5.0

decreased cell proliferation ( J:386262 )

• in embryos at E5.0

decreased embryonic epiblast cell proliferation ( J:386262 )

• decreased proliferation coinciding with an increase in gamma H2AX foci indicating premature senescence

early cellular replicative senescence ( J:386262 )

• in the epiblast at E5.0

• premature senescence is seen in visceral endoderm and extraembryonic ectoderm

abnormal lysosome physiology ( J:386262 )

• impaired acidification indicated by elevated pH and reduced proteolytic activity

• reduction of lysosomal pH rescues premature senescence and extend survival of embryos by 2 days

homeostasis/metabolism

impaired autophagy ( J:386262 )

• blockade in autophagic flux

• following infection with a reporter adenovirus embryos show an increase in autophagosomes

growth/size/body

decreased embryo size ( J:386262 )

• at E5.5

• at E5.0 the total number of cells in all three lineages (epiblast, visceral endoderm, and extraembryonic ectoderm) is reduced