Phenotypes associated with this allele

• Allele Symbol: Coxfa4l3^em1Ms
• Allele Name: endonuclease-mediated mutation 1, National Institute of Genetics, Mishima
• Allele ID: MGI:8347484
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Coxfa4l3^em1Ms/Coxfa4l3^em1Ms	C57BL/6J-Coxfa4l3^em1Ms	MGI:8350407

Genotype
MGI:8350407

hm1

• Allelic Composition: Coxfa4l3^em1Ms/Coxfa4l3^em1Ms
• Genetic Background: C57BL/6J-Coxfa4l3^em1Ms

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:386425 )

♂N • adult male mice are fertile with no major alterations in testicular morphology and histology relative to wild-type controls; testicular cells show no changes in intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, and ATP levels (under FCCP-untreated conditions), suggesting normal mitochondrial function in testes

decreased sperm progressive motility ( J:386425 )

♂ • CASA analysis indicates a significant decrease in sperm progressive motility

asthenozoospermia ( J:386425 )

♂ • CASA analysis indicates a significant decrease in the % of motile spermatozoa relative to wild-type controls; although the amplitude of lateral head displacement (ALH) is relatively normal, all other tested motility parameters, including progressive motility, are significantly decreased following capacitation

homeostasis/metabolism

decreased cellular ATP level ( J:386425 )

♂ • under glycolysis-independent conditions, sperm show a significant decrease in ATP levels relative to wild-type sperm

decreased mitochondria enzyme/coenzyme activity ( J:386425 )

♂ • testicular mitochondria show a significant decrease in electron transport complex IV activity relative to wild-type mitochondria

♂ • however, complex I and complex II activities are relatively normal in testes

decreased cytochrome-c oxidase activity ( J:386425 )

♂ • testicular mitochondria show a significant decrease in electron transport complex IV activity relative to wild-type mitochondria

♂ • however, complex I and complex II activities are relatively normal in testes

cellular

decreased cellular ATP level ( J:386425 )

♂ • under glycolysis-independent conditions, sperm show a significant decrease in ATP levels relative to wild-type sperm

decreased sperm progressive motility ( J:386425 )

♂ • CASA analysis indicates a significant decrease in sperm progressive motility

asthenozoospermia ( J:386425 )

♂ • CASA analysis indicates a significant decrease in the % of motile spermatozoa relative to wild-type controls; although the amplitude of lateral head displacement (ALH) is relatively normal, all other tested motility parameters, including progressive motility, are significantly decreased following capacitation

abnormal mitochondrial physiology ( J:386425 )

♂ • after treatment with FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), testicular mitochondria show a significant reduction in maximal respiratory capacity relative to FCCP-treated wild-type mitochondria

♂ • however, basal respiration is relatively normal in untreated testis mitochondria

decreased mitochondria enzyme/coenzyme activity ( J:386425 )

♂ • testicular mitochondria show a significant decrease in electron transport complex IV activity relative to wild-type mitochondria

♂ • however, complex I and complex II activities are relatively normal in testes