Phenotypes associated with this allele

• Allele Symbol: Gpr158^tm1.1Jdw
• Allele Name: targeted mutation 1.1, Joris de Wit
• Allele ID: MGI:8318985
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw	involves: C57BL/6	MGI:8324972
cn2	Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw Rorb^tm1.1(cre)Hze/Rorb^+	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * C57BL/6NCrl	MGI:8324945
cn3	Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw Plcxd2^tm1.1Jdw/Plcxd2^tm1.1Jdw	involves: C57BL/6	MGI:8324973

Genotype
MGI:8324972

cn1

• Allelic Composition: Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal dendritic spine morphology ( J:372545 )

• mice injected with adenoviruses (AAV) expressing TRE-cre and SYN-DIO-GFP-IRES-tTA in the barrel cortex to induce expression of cre and GFP in a small population of L4 cortical neurons, show a shift towards an immature dendritic spine morphology, with reduced mature mushroom-type spines and increased immature filopodia-type protrusions

• mice injected with AAV-TRE-cre and AAV-SYN-DIO-GFP-IRES-tTA in the barrel cortex to induce expression of cre and GFP in a wide population of L4 cortical neurons, show a strong reduction in the number of spine apparatus-bearing spines; the fraction of mushroom-type dendritic spines that contain spinus apparatus is halved compared to controls

Genotype
MGI:8324945

cn2

• Allelic Composition: Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw; Rorb^{tm1.1(cre)Hze}/Rorb⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * C57BL/6NCrl

nervous system

abnormal dendritic spine morphology ( J:372545 )

• dendritic spines of neurons are shifted toward a less mature phenotype, with a decrease in the fraction of mature mushroom-type spines and a concomitant increase in immature filopodia-type protrusions indicating attenuation of dendritic spin maturation

• however, dendritic spine density is unaffected

decreased miniature excitatory postsynaptic current frequency ( J:372545 )

• acute cortical sections from P15-21 mice show a strong reduction in the frequency of miniature excitatory post-synaptic currents (mEPSCs)

• however, amplitude and decay time of mEPSCs are not affected

Genotype
MGI:8324973

cn3

• Allelic Composition: Gpr158^tm1.1Jdw/Gpr158^tm1.1Jdw; Plcxd2^tm1.1Jdw/Plcxd2^tm1.1Jdw
• Genetic Background: involves: C57BL/6

nervous system

nervous system phenotype ( J:372545 )

N • mice injected with adenoviruses (AAV) expressing TRE-cre and SYN-DIO-GFP-IRES-tTA in the barrel cortex to induce expression of cre and GFP in L4 cortical neurons show rescue of the loss of mature mushroom-type spines and increase in immature filopodia-type protrusions in Gpr158 conditional knock-out neurons and show rescue of the spine apparatus content of dendritic spines to that seen in wild-type mice