Phenotypes associated with this allele

• Allele Symbol: Ace2^em1(ACE2)Dfl
• Allele Name: endonuclease-mediated mutation 1, Dongfang Liu
• Allele ID: MGI:8250890
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ace2^em1(ACE2)Dfl/Ace2^em1(ACE2)Dfl	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Ace2^em1(ACE2)Dfl	MGI:8250895
ot2	Ace2^em1(ACE2)Dfl/Y	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Ace2^em1(ACE2)Dfl	MGI:8250894

Genotype
MGI:8250895

hm1

• Allelic Composition: Ace2^em1(ACE2)Dfl/Ace2^em1(ACE2)Dfl
• Genetic Background: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Ace2^em1(ACE2)Dfl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to Coronaviridae infection ( J:369237 )

♀ • mice inoculated with SARS-Cov-2 USA-WA1/2020 virus exhibit ruffling of the fur and significant body weight loss and body weights of infected mice do not return to initial weight levels by the end of day 7

♀ • mice develop a significant viral burden that persists in the lungs for at least 7 days post-infection, with a nearly 100,000-fold increase in presence of SARS-CoV-2 nucleocapsid RNA in the lungs and show an increase in SARS-CoV-2 Spike protein in lung tissues 7 days post-infection

♀ • an increase in total CD45+ lymphocytes in the lungs are seen 48 hours after SARS-CoV-2 infection, however no significant inflammation-induced pathology in the lungs is seen, most likely due to the defective innate and humoral immune response systems in the NSG background

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
COVID-19		DOID:0080600		J:369237

Genotype
MGI:8250894

ot2

• Allelic Composition: Ace2^em1(ACE2)Dfl/Y
• Genetic Background: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Ace2^em1(ACE2)Dfl

immune system

increased susceptibility to Coronaviridae infection ( J:369237 )

♂ • mice inoculated with SARS-Cov-2 USA-WA1/2020 virus exhibit ruffling of the fur and significant body weight loss and body weights of infected mice do not return to initial weight levels by the end of day 7

♂ • mice develop a significant viral burden that persists in the lungs for at least 7 days post-infection, with a nearly 100,000-fold increase in presence of SARS-CoV-2 nucleocapsid RNA in the lungs and show an increase in SARS-CoV-2 Spike protein in lung tissues 7 days post-infection

♂ • an increase in total CD45+ lymphocytes in the lungs are seen 48 hours after SARS-CoV-2 infection, however no significant inflammation-induced pathology in the lungs is seen, most likely due to the defective innate and humoral immune response systems in the NSG background

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
COVID-19		DOID:0080600		J:369237