Analysis Tools
nervous system
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• in cre treated primary neurons, active zone length, postsynaptic density length, and vesicle cluster area are significantly decreased
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• average synaptic vesicle size (defined as all clear core vesicles smaller than 50 nm) is reduced by 5% in cultured primary neurons treated with cre
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• in null vesicles, after cre treatment, the number of docked vesicles is increased but the total number of vesicles is not altered
• when normalized to the active zone length the density of docked vesicles increases by 50%
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• following viral cre treatment, primary cultured single hippocampal neurons during a train of 100 action potentials at 40 Hz show highly increased charge
• the average charge between pulse 90 and 100 is increased by 110% in null synapses
• however, no effect is seen on single EPSCs, mEPSCs, or the size and refilling of the readily releasable vesicle pool
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cellular
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• average synaptic vesicle size (defined as all clear core vesicles smaller than 50 nm) is reduced by 5% in cultured primary neurons treated with cre
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• in null vesicles, after cre treatment, the number of docked vesicles is increased but the total number of vesicles is not altered
• when normalized to the active zone length the density of docked vesicles increases by 50%
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