Analysis Tools
mortality/aging
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• no homozygotes are born from heterozygous intercrosses; only 14.29% and 16.67% homozygous embryos are identified at E6.5 and E7.5, respectively; all 6 identified E7.5 homozygous embryos are highly degenerated and nonviable
• supplementing extraembryonic tissue with functional DIS3L via construction of chimeric embryos does not rescue the embryonic lethality
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embryo
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• at E7.5, all 6 identified homozygous embryos are highly degenerated and nonviable
• however, overall preimplantation development is normal; blastocysts show no significant changes in mean total cell count or percentage ratio of all three cell lineages (trophectoderm, epiblast, and primitive endoderm) relative to wild-type controls
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• at E6.5, two of 3 identified homozygous embryos are smaller and deformed relative to wild-type controls
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• at E6.5, two of 3 identified homozygous embryos are smaller than wild-type controls
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growth/size/body
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• at E6.5, two of 3 identified homozygous embryos are smaller than wild-type controls
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homeostasis/metabolism
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• preimpantation embryos show a decrease in global protein synthesis -- possibly the effect of translation stress response pathways triggered by accumulation of aberrant transcripts
• however, preimplantation embryos are still able to produce functional embryonic stem (ES) cells from the inner cell mass, albeit with reduced efficiency; RNA-seq analysis shows no major changes at the transcriptome level for both ES cells and blastocysts
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cellular
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• preimpantation embryos show a decrease in global protein synthesis -- possibly the effect of translation stress response pathways triggered by accumulation of aberrant transcripts
• however, preimplantation embryos are still able to produce functional embryonic stem (ES) cells from the inner cell mass, albeit with reduced efficiency; RNA-seq analysis shows no major changes at the transcriptome level for both ES cells and blastocysts
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